Self-calibrating viscosity probes: design and subcellular localization.

Synthesis and evaluation of self-calibrating ratiometric viscosity sensors.

We describe the design, synthesis and fluorescent profile of a family of self-calibrating dyes that provide ratiometric measurements of fluid viscosity. The design is based on covalently linking a primary fluorophore (reference) that displays a viscosity-independent fluorescence emission with a secondary fluorophore (sensor) that exhibits a viscosity-sensitive fluorescence emission. Characteriz...

Evidence for an association between Wnt-independent -catenin intracellular localization and ovarian apoptotic events in normal and PCO-induced rat ovary

The association of secreted frizzled related protein type 4 (Sfrp4) as an antagonist of Wnt mole-cules in apoptotic events has been reported previously. Moreover, its increased expression has been reported in the ovary of women with polycystic ovary (PCO). We have demonstrated in-creased Sfrp4 in PCO-induced rat ovary related to an increased number of apoptotic follicles showing nuclear ?cateni...

Unravelling the correlation between metal induced aggregation and cellular uptake/subcellular localization of Znsalen: an overlooked rule for design of luminescent metal probes.

Unravelling the unique effects of metal coordination on biological behaviours is of importance to design metal based therapeutic and diagnostic agents. In this work, we chose luminescent Znsalen (ZnL1 ) as a case study to demonstrate that metal induced aggregation arising from the intermolecular Zn···O interaction influences its cellular uptake and subcellular localization. Comparative studies ...

Unravelling the correlation between metal induced aggregation and cellular uptake/subcellular localization of Znsalen: an overlooked rule for design of luminescent metal probes† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4sc03824j Click here for additional data file.

Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes

The investigation of the subcellular localization, dynamics and interaction of proteins and protein complexes in prokaryotes is complicated by the small size of the cells. Super-resolution microscopy (SRM) comprise various new techniques that allow light microscopy with a resolution that can be up to ten-fold higher than conventional light microscopy. Application of SRM techniques to living pro...